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Sp2/0-Ag14; Non-secreting Hybridoma; Mouse (Mus musculus) · Product Code · Product Categories · Biosafety Level · Product Type · Cell type · Organism · Tissue · Product. Pool the three T flasks of SP2/0 cells into one flask. Count cells. You will want about 1× cells/ml. Aliquot 4× SP2/0 cells in a 50 mL centrifuge tube. Anyone know what's wrong with my SP2/0????? This strange Sp2/0 is surrounding my hybridoma cells in EVERY wells and I am suspecting it may be.

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Anyone know what's wrong with my SP2/0????? This strange Sp2/0 is surrounding my hybridoma cells in EVERY wells and I am suspecting it may be. CHO, NS0 and Sp2/0 cell lines. Maintaining biosimilarity is critical for regulatory approval of biosimilar products and Abzena can help you achieve this by. runs with Sp2/0 myeloma cells. These findings are of relevance for the safety of protein products derived from myeloma cells. KEYWORDS: myeloma cell line.

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Sp2/0-Ag14 cells can be used as fusion partners for B cells in the production of hybridomas. References: Shulman M et al. A better cell line for making. Sp2/0-Ag14 is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Among the cell lines derived from MOPC21, the SP2/0 (hybridoma), P3-XAg (myeloma) and NS0 (myeloma) cell lines are frequently used as fusion.